Имеется собственный автомобиль

Права категории B

AWARDS 2016 Award of the Ministry of the Education and Science, Republic of Buryatia, Russia 2012 The Best Young Scientist of Ulan-Ude City. Administration of Ulan-Ude City (Republic of Buryatia, Russia). PROFESSIONAL MEMBERSHIPS European Association of Fish Pathologists European Federation of Parasitologists PROJECTS PARTICIPATED IN 2016-2018 Study of the structural and molecular basis of immunomodulation in Parasite-Host systems “Cestodes – Fishes” The Russian Foundation for Basic Research. 2015-2017 Study of the structural and molecular basis of host-parasite relationships at different stages of the life cycle of the tapeworm case studies on the units Diphyllobothriidea, Trypanorhyncha and Diphyllidea. The Russian Foundation for Basic Research. 2012-2014 Immunomorphological investigation of coadaptation mechanisms in the system “Tapeworm Diphyllobothrium dendriticum – Baikal omul Coregonus migratorius” The Russian Foundation for Basic Research. 2012 Investigation of the Diphyllobothriidae parasites species molecular genetics diversity in the organism of Salmonoidei fishes of lakes Baikal and Khubsugul. The Russian Foundation for Basic Research 2011 Do Cestodes produce neuromediators and prostaglandins to manipulate immune responses of their fish hosts? Deutscher Akademischer Austausch Dienst (DAAD) 2010 Immunohistochemical investigations of immunomodulators in Cestoda helminth. The Russian Foundation for Basic Research. 2008-2010 Immunological and micromorphological aspects of relationship of tapeworm Diphyllobothrium dendriticum (Cestoda: Pseudophyllidae) with supplementary and definitive hosts. The Russian Foundation for Basic Research 2007 Immunological and morphofunctional mechanisms of relationship in system Diphyllobothrium dendriticum – definitive host. Buryat State University 2006 Immunological and morphofunctional aspects of pathogenesis of diphyllobothriasis “B” in Baikal nature pesthole. Buryat State University. 2005 Research of Baikal Nerpa immune status in system of Lake Baikal Biomonitoring. Iindividual grant of the East Siberian State Technological University Priority within the frame of the program “Science for young scientists”. 2003 Protection of Lake Baikal and Baikal Natural Territory – Health Monitoring of Baikal Seal Population. Russian Ministry of Natural Resources. 2003 Reception of histomorphological indexes of Baikal Nerpa immune organs Iindividual grant of Federal Science Program “Integration”. 2002 Carrying out of Expedition on Studying of Baikal Endemics. International Project of Siberian Branch of Russian Academy of Sciences with participating of Prof. Otto Grahl-Nielsen (Norway, University of Bergen) and Prof. Reijo Kakela (Finland, University of Jonsuu). RESEARCH EXPERIENCE The main goal of current research is to understand cell, biochemical and molecular bases of relations between parasites and host immune system. I used model systems: Diphyllobothrium dendriticum – baikal omul Coregonus migratorius, Ligula interrupta – crucian carp Carassius gibelio, Schistocephalus solidus – three-spined stickleback Gasterosteus aculeatus, D. dendriticum – hamster Mesocricetus auratus, Flavobacterium psychrophilum – rainbow trout Oncorhynchus mykiss. CELL, BIOCHEMICAL AND MOLECULAR PARASITOLOGY 2010-2017. Investigation of mechanisms of Cestodes regulation of immune response in their fish hosts. Results: Immunofluorescence and confocal microscopy. Distribution of immune regulatory molecules: prostaglandins E2, D2; neuroactive substances GABA, 5-HT, FMRF-amide has been investigated in D. dendriticum and L. interrupta plerocercoids. PGE2 localized in cytons of neurons, flame cells, the apical terminals of the frontal glands and sensory organsin the tegument. PGD2 occurred in the muscle fibers and flame cells. Thereby, prostaglandins E2, D2 may be secreted by parasites into host tissues through nervous and excretory systems and frontal glands; neuroactive substances – through the nervous system. High-performance liquid chromatography. D. dendriticum secretes prostaglandins E2, D2 in the incubating medium after influence of blood serum from its fish hosts. Maximum of secretion is at 12 h post incubation. Scanning and transmission electron microscopy. D. dendriticum and L. interrupta plerocercoids increased their secretion in tegument and free nerve endings after influence of blood serum from its fish hosts. Maximum of secretion is at 6-12 h post incubation. Secretion mechanisms are: apocrine, merocrine, going out of vesicles from the surface of nerve endings. The number of rhabdiform organelle, discal bodies, vesicles and vacuoles increased in the distal cytoplasm of the tegument after incubation in blood serum. 2012-2017. Molecular genetics investigations of Diphyllobothrium genius in fishes from the Republic of Buryatia (Russia) and Mongolia. Preliminary results: PCR. Obtaining of ITS1 and CO1 genes fragments sequences from 70 Diphyllobothrium samples. Draft phylogenetic trees were constructed. 2016-2017. Study of D. dendriticum proteome to find immunoregulating peptides. Preliminary results: D. dendriticum proteins were separated using 2D gel electrophoresis. PARASITE IMMUNOLOGY 2011. Influence of Cestodes immune regulatory molecules on fish immunity. Cell culture, flow cytometry, microplate reader. In short term (2 h) head kidney leucocytes (HKL) cultures PGE2 did not change the total numbers of live HKL, but the production of reactive oxygen species (ROS) decreased significantly with high PGE2 concentrations. In long-term (96 h) cultures high PGE2 concentrations induced a sharp decrease of leucocytes viability, while low and intermediate concentrations of PGE2 caused elevated leucocyte viability compared to controls. This coincided with reduced ROS production in cultures with high PGE2 and elevated ROS production in cultures with low PGE2. Granulocyte to lymphocyte ratios increased with high PGE2 concentrations alone and in combination with S. solidus antigens and LPS, most prominently with HKL from S. solidus infected sticklebacks. 5-HT and GABA neiromediators have less strong effect as compared with PGE2. At culture incubated for 96 h, they increase respiratory burst, similarly to extracts of S. solidus and LPS. But immediately they decrease respiratory burst, similarly to PG E2. 2005-2014. Changes of microanatomical structure and cell composition of immune organs after Cestodes invasion. Results: Light microscopy. Neutrophils proliferation and differentiation occurs in Baikal omul pronephros after D. dendriticum invasion. Neutrophil migrate from omul pronephros and spleen to parasite localisation. B-lymphocyte migrate from pronephros to spleen. The number of neutrophilic metamyelocytes and prolymphocites increases in the crucian head kidney after L. interrupta invasion. Neutrophils migrate to blood and the spleen. B-lymphocyte migrate from pronepros to spleen. It is shown decreasing of the number of blasts and acidophilic granulocytes in head kidney and basophilic granulocytes – in mesonephric kidney. It has shown decreasing of plasma cells in medulla sinuses and increasing of big lymphocytes in paracortical zone of hamster’s mesenteric lymph nodes after D. dendriticum invasion. The number of mast cells increases more then 6 times in duodenum. The degree of mast cell granule saturation decreases. The number of degranulated mast cells increases more than 17 times. In summary, against the background activation of any nonspecific and adaptive immunity, we observed suppression of other host immune reactions. 2015. Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with F. psychrophilum in resistant and susceptible lines of rainbow trout (O. mykiss). Results: GeXP multiplex RT-PCR assay. Spleen tissue was sampled at 6 h, 24 h, 48 h and 144 h post-challenge and pathogen load quantified by qPCR. Transcript abundance of cytokine genes tnfa1, tnfa2, tnfa3, il1b1, il1b2, il11a; acute phase response genes saa and drtp1; and putative cytokine receptors il1r1-like-b, il1r2, tnfrsf1a, tnfrsf9, tnfrsf1a-like-b increased following challenge while the transcript abundance of il1r-like-1 and tnfrsf1a-like-a decreased compared to PBS-injected line-matched control fish. Principal component analysis identified transcript levels of genes il1r-like- 1 and tnfrsf1a-like-a as exhibiting differential expression between genetic lines. List of Publications PUBLICATIONS IN PEER-REVIEWED JOURNALS Y. Zheng; X. Guo; M. Su; X. Chen; X. Jin; J. Ding; Z. Wang; X. Bo; M. Ayaz; I. Kutyrev; W. Jia; X. Zhang; J. Zhang. Identification of emu-TegP11, an EF-hand domain-containing tegumental protein of Echinococcus multilocularis // Veterinary Parasitology. – 2018 – In Press. Kutyrev I.A., N.M. Biserova, D.N. Olennikov, Z.V. Korneva, O.E. Mazur. Prostaglandins E2 and D2 – regulators of host immunity in the model parasite Diphyllobothrium dendriticum: an immunocytochemical and biochemical study // Mol. Bioch. Par. – 2017а. – P. 33-45. Kutyrev I.A., B. Cleveland, T. Leeds, G.D. Wiens Dataset of proinflammatory cytokine and cytokine receptor gene expression in rainbow trout (Oncorhynchus mykiss) measured using a novel GeXP multiplex, RT-PCR assay // Data in Brief. – 2017b. – DOI:10.1016/j.dib.2017.02.014. Kutyrev I.A., B. Cleveland, T. Leeds, G.D. Wiens Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss) // Fish Shellfish Immunol. – 2016. – V. 58. – P. 542-553. Kutyrev I.A., Franke F., Büscher J., Kurtz J., Scharsack J.P. In vitro effects of prostaglandin E2 on leucocytes from sticklebacks (Gasterosteus aculeatus) infected and not infected with the cestode Schistocephalus solidus // Fish Shellfish Immunol. 2014. V. 41. P. 473-481. Biserova N.M., Kutyrev I.A., Jensen K. GABA in the nervous system of the Cestodes Diphyllobothrium dendriticum (Diphyllobothriidea) and Caryophyllaeus laticeps (Caryophyllidea), with comparative analysis of muscle innervation // J. Parasitol., 100(4), 2014. P. 411-421. Biserova N.M., Kutyrev I.A. Localization of prostaglandin E2, γ-aminobutyric acid, and other potential immunomodulators in the plerocercoid Diphyllobothrium dendriticum (Cestoda) // Biology Bulletin, 2014, Vol. 41, No. 3, pp. 242–250. Kutyrev I.A., Scharsack J.P., Biserova N.M., Kurtz J. Prostaglandin E2 as a gull tapeworm potential immunomodulator // The Journal of East Siberian Scientific Center of Russian Academy of Medical Sciences. 2012. С. 245-249. Averina E.S., Kutyrev I.A. Perspectives of using of marine and freshwater hydrobionts oils for development of drug delivery systems // Biotechnol. Adv. 2011. Vol. 29(5). P. 548-557. Kutyrev I.A., Pronin N.M., and Dugarov Zh.N. Composition of Leucocytes of the Head Kidney of the Crucian Carp (Carassius auratus gibelio, Cypriniformes: Cyprinidae) as Affected by Invasion of Cestode Digramma interrupta (Cestoda; Pseudophyllidea). Biology Bulletin, Wilmington, USA, 2011, Vol. 38, No. 6, pp. 653–657. Biserova N.M., Kutyrev I.A., Malakhov V.V. Tapeworm Diphyllobothrium dendriticum (Cestoda) produces prostaglandin E2 – regulator of host immunity // Doklady of Russian Academy of Sciences, 2011, V. 441, №1 Kutyrev I.A., Pronina S.V. Hamster duodenum mast cells composition changes during experimental invasion with Diphyllobothrium dendriticum (Cestoda: Pseudophyllidae) // Morphology. – 2010. – V. 137. - № 2. – P. 44 – 47. Pronina S.V., Kutyrev I.A., Tolochko L.V., Mazur O.E., Pronin N.M. Morphofunctional and immunological aspects of diphillobothriasis pathogenesis experimentally causing by Diphyllobothrium dendriticum (Pseudophyllidea: Diphyllobothriidae) in hamster // Bulletin of Buryat State Agriculture Academy. – 2010. - № 3. – P. 25-31. Pronin N.M., Pronina S.V., Kutyrev I.A. Baikal natural diphyllobothriasis structure and relationship between Diphyllobothrium dendriticum and definitive hosts // Bulletin of Irkutsk State University. Series Biology and Ecology. – 2009. - №1. – P. 53-56. Kutyrev I.A. Changes of hamster mesenteric lymph nodes cell composition during experimental invasion with Diphyllobothrium dendriticum (Cestoda: Pseudophyllidae) // Bulletin of Buryat State Agriculture academy. - 2009. - №4. – pp. 17-21. Kutyrev I.A., G.P. Lamazhapova, S.D. Zhamsaranova. Structural organization of mesenteric lymphatic nodes of Baikal nerpa Pusa sibirica Gmel. in postnatal ontogeny // Biology Bulletin. – Wilmington, USA. - 2008. - № 4. – pp. 389-393. Kutyrev I.A., G.P. Lamazhapova, S.D. Zhamsaranova. Structural organization of mesenteric lymphatic nodes of Baikal nerpa Pusa sibirica Gmel. in postnatal ontogeny // Proceedings of the Russian Academy of Sciences. Series Biology. - 2008. - № 4. –pp. 453-458. Kutyrev I.A., G.P. Lamazhapova, S.D. Zhamsaranova. Cell composition of cortex of Baikal seal mesenteric lymph nodes in postnatal ontogeny // Morphology. - 2008. – T. 134, No 6. - P. 38-41. Kutyrev I. A., B. B. Khandazhapova, G. P. Lamazhapova, S. D. Zhamsaranova, V. Ts. Tsydypov. Histomorphological indexes of mesenteric lymphatic nodes of Baikal seal (Pusa sibirica) when finding microorganisms in intestine which are pathogenic for white mice and in the absence of microorganisms // Bulletin of Buryat State University. Series “Biology. Geography. Chemistry”. - 2007. –№ 3. – P. 301-304. Kutyrev I.A., G.P. Lamazhapova, L.M. Erofeeva, S.D. Zhamsaranova. Comparison of microanatomical and cytological characteristics of mesenteric lymphatic node of adolescents and adult individuals of Baikal seal (Pusa sibirica) // Zoological Journal. – 2006. – Т. 85, № 7. – P. 886-892. Erofeeva L.M., S.D. Zhamsaranova, I.A. Kutyrev, G.P. Lamazhapova, M.R. Sapin. Species features of morphology and cytoarchitectonics of mesenteric lymph nodes of pubertal Baikal Nerpa // Bulletin of new medical technologies - 2004. – T. XI. - № 3. – P. 18-20. Kutyrev I.A., L.M. Erofeeva, M.R. Sapin, S.D. Zhamsaranova, G.P. Lamazhapova. Age changes of mesenteric lymph nodes morphology of the Baikal Nerpa // Morphological bulletin. - 2004. - № 1-2 - P. 58. G.P. Lamazhapova, I.A. Kutyrev, S.D. Zhamsaranova. Some features of thymus and group lymphoid nodules morphogenesis of Baikal Nerpa in age aspect // The bulletin of Russian Academy of Medical Sciences. – 2003- T.4. - № 5. - P. 30 CONTRIBUTION TO BOOKS Kutyrev I.A., Lamazhapova G.P., Zhamsaranova S.D. Mesenteric lymphatic nodes of the Baikal Nerpa: histomorphology in postnatal ontogeny. – Berlin: LAP Lambert Academic Publishing, 2011. – 176 pp. Lamazhapova G.P., Grigorenko D.E., Aminova G.G., Erofeeva L.M., Zhamsaranova S.D., Kutyrev I.A., Donditov E.V. Organs of Baikal Nerpa (Pusa sibirica Gmel.) immune system. – Moscow: “Sputnik+” publishing, 2011– 133 pp. Kutyrev I.A. Water Mammals. Baikal Nerpa. Muskrat. In: Baikal: Nature and People. Encyclopaedic Manual. Ulan-Ude: Publishing of BSC SB RAS, Russia, 2008. – pp. 142-144. Kutyrev I.A., N.M. Pronin, L.S. Imikhelova, E.A. Petrov, E.A. Kuzmina. Baikal Nerpa: the Passport and the Bibliography. – Ulan-Ude: Publishing of BSC SB RAS, Russia, 2006. - 92 p. PARTICIPATION IN INTERNATIONAL CONFERENCES Kutyrev I.A., Scharsack J.P., Biserova N.M., Kurtz J.. Prostaglandin E2 as a Cestodes immunomodulator // XI European Multicolloquium of Parasitology, Cluj-Napoca, Romania: 25 – 29 July 2012. Kutyrev I.A., Pronina S.V., Pronin N.M. Immune response of Baikal omul to diphyllobothriasis // Gordon Research Conference on Immunochemistry and Immunobiology, Les Diablerets, Switzerland, 16-22 May 2010. Kutyrev I.A., Pronina S.V., Pronin N.M. Influence of Diphyllobothrium dendriticum invasion on cell composition of hamster’s mesenteric lymph node and duodenum // The Impact of the Environment on Innate Immunity: The threat of Diseases: ESF-FWF Conference in Partership with LFUI. – Obergurgl, Austria, 4-9 May 2009. – P. 42.